FastQCFastQC Report
Wed 8 May 2024
EGAF00001682379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946210
Total Bases220.9 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCC2017336.847203695595358Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGC579641.9674089762779976Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCG133760.4540070123989804Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACA127590.43306485281089946Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTG80200.27221413273324035TruSeq Adapter, Index 2 (95% over 24bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATC62300.21145811058953706Illumina RNA RT Primer (95% over 22bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTC60320.20473761205073637Illumina RNA RT Primer (95% over 22bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATG57470.19506416718428082Illumina RNA RT Primer (95% over 22bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA51250.17395229803713924Illumina RNA RT Primer (95% over 22bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA49050.1664850774384718Illumina RNA RT Primer (95% over 22bp)
CCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTAT44190.14998930829777918Illumina RNA RT Primer (95% over 22bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA41840.14201295902192987Illumina RNA RT Primer (95% over 22bp)
ACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTAT40670.13804175533991125Illumina RNA RT Primer (95% over 22bp)
CACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA37730.12806283326714663Illumina RNA RT Primer (95% over 22bp)
ATCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA35020.1188645751660608Illumina RNA RT Primer (95% over 22bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATG34990.11876274943062443Illumina RNA RT Primer (95% over 22bp)
AACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA30770.10443926264590779Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)