FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946210
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1856796.302300243363508TruSeq Adapter, Index 2 (96% over 28bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG526911.7884332752926642RNA PCR Primer, Index 21 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA130600.443281368266349RNA PCR Primer, Index 21 (96% over 27bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCT115690.39267397775447105RNA PCR Primer, Index 21 (96% over 25bp)
CGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG75080.25483587388543244TruSeq Adapter, Index 2 (96% over 28bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAA60530.20545039219879097RNA PCR Primer, Index 21 (96% over 27bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA58720.1993069061607964RNA PCR Primer, Index 21 (96% over 27bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG50800.17242491200559362RNA PCR Primer, Index 21 (96% over 27bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA47430.1609864877249076RNA PCR Primer, Index 21 (96% over 27bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA45660.15497876933416152RNA PCR Primer, Index 21 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGT41800.14187719137468138TruSeq Adapter, Index 2 (96% over 28bp)
CCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAA41690.141503830344748RNA PCR Primer, Index 21 (96% over 27bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA39210.13308623621534107RNA PCR Primer, Index 21 (96% over 27bp)
ACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAA38190.12962416121050432RNA PCR Primer, Index 21 (96% over 27bp)
CACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA34910.11849121413612743RNA PCR Primer, Index 21 (96% over 27bp)
ATCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAA32760.11119370309652062RNA PCR Primer, Index 21 (96% over 27bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG30900.10488050749946543RNA PCR Primer, Index 21 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)