FastQCFastQC Report
Wed 8 May 2024
EGAF00001682380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901015
Total Bases217.5 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCC2000866.897103255239976Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGC574481.9802724218937164Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCG130720.4506009103710253Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACA126180.4349512153504894Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCGTCTTCTG76800.26473492898175294TruSeq Adapter, Index 2 (95% over 24bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATC61200.21096064653233437Illumina RNA RT Primer (95% over 22bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTC57360.19772390008324672Illumina RNA RT Primer (95% over 22bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATG56760.19565565845057678Illumina RNA RT Primer (95% over 22bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA49230.16969922596056897Illumina RNA RT Primer (95% over 22bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA48660.16773439640953253Illumina RNA RT Primer (95% over 22bp)
CCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTAT41540.14319126236851584Illumina RNA RT Primer (95% over 22bp)
ACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTAT40190.13853771869500847Illumina RNA RT Primer (95% over 22bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA39820.13726230302152867Illumina RNA RT Primer (95% over 22bp)
CACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA37780.13023028147045088Illumina RNA RT Primer (95% over 22bp)
ATCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA35170.1212334303683366Illumina RNA RT Primer (95% over 22bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATG35050.1208197820418026Illumina RNA RT Primer (95% over 22bp)
AACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTA30640.10561820604167851Illumina RNA RT Primer (95% over 22bp)
GAATTCTCGGGTGCCAAGTCGACGTACGATCACATCTATCTCGTATGCCG30190.10406702481717606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)