FastQCFastQC Report
Wed 8 May 2024
EGAF00001682381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912836
Total Bases218.4 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCC2283017.837756742913092Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGC598662.055247875266579Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCG99380.3411795240102773Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAG90030.30908022284811093Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTG83790.28765780153774534RNA PCR Primer, Index 41 (96% over 25bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATG59690.20492056538713474Illumina RNA RT Primer (95% over 22bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTC44690.15342436031414058Illumina RNA RT Primer (95% over 22bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGG43740.15016293399285097Illumina RNA RT Primer (95% over 22bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA42890.14724481570538128Illumina RNA RT Primer (95% over 22bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATC41410.14216385680484586Illumina RNA RT Primer (95% over 22bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATG36650.12582239439501572Illumina RNA RT Primer (95% over 22bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA34550.11861292568479653Illumina RNA RT Primer (95% over 22bp)
CCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTAT34340.11789197881377461Illumina RNA RT Primer (95% over 22bp)
CTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTAT34160.11727402435289869Illumina RNA RT Primer (95% over 22bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA33670.11559181498718087Illumina RNA RT Primer (95% over 22bp)
GAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCG32620.11198708063207129No Hit
CCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACAT31160.10697478333829985Illumina RNA RT Primer (95% over 22bp)
ACTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTAT31050.1065971445010979Illumina RNA RT Primer (95% over 22bp)
GCACCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA30810.10577320521993Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)