FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912836
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG2094227.189625505864387RNA PCR Primer, Index 41 (96% over 29bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG540271.8547903143191034RNA PCR Primer, Index 41 (96% over 29bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA96110.32995335130436454RNA PCR Primer, Index 41 (96% over 29bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCT79210.2719342935887911RNA PCR Primer, Index 41 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG78160.26832955923368157RNA PCR Primer, Index 41 (96% over 29bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG52020.17858883919314372RNA PCR Primer, Index 41 (96% over 29bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAA43130.1480687549865492RNA PCR Primer, Index 41 (96% over 29bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTT40190.13797549879224233RNA PCR Primer, Index 41 (96% over 28bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAA39830.13673958987049048RNA PCR Primer, Index 41 (96% over 29bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAA38680.1327915474815609RNA PCR Primer, Index 41 (96% over 29bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGT35800.12290427610754605RNA PCR Primer, Index 41 (96% over 29bp)
CCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA33370.114561890885721RNA PCR Primer, Index 41 (96% over 29bp)
CTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA33100.11363495919440711RNA PCR Primer, Index 41 (96% over 29bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG32100.1102018788562075RNA PCR Primer, Index 41 (96% over 29bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAA31360.10766139940593979RNA PCR Primer, Index 41 (96% over 29bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAA31100.10676879851800788RNA PCR Primer, Index 41 (96% over 29bp)
GAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG30450.10453729629817812RNA PCR Primer, Index 41 (96% over 29bp)
ACTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA29920.10271776371893235RNA PCR Primer, Index 41 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)