FastQCFastQC Report
Wed 8 May 2024
EGAF00001682382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846582
Total Bases213.4 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCC2243427.881100913305852Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGC589952.0724855282581003Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCG95300.33478747494363414Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAG90110.3165550825516356Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTG80290.28205756939375015RNA PCR Primer, Index 41 (96% over 25bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATG58580.20579066403145946Illumina RNA RT Primer (95% over 22bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTC44940.1578735479954556Illumina RNA RT Primer (95% over 22bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGG41820.1469130346499767Illumina RNA RT Primer (95% over 22bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA40560.14248667349122562Illumina RNA RT Primer (95% over 22bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATC39290.13802518248200826Illumina RNA RT Primer (95% over 22bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATG34070.11968740053861088Illumina RNA RT Primer (95% over 22bp)
CTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTAT33430.11743909010876903Illumina RNA RT Primer (95% over 22bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA33360.1171931811555051Illumina RNA RT Primer (95% over 22bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA33220.11670136324897719Illumina RNA RT Primer (95% over 22bp)
CCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTAT33190.11659597369757836Illumina RNA RT Primer (95% over 22bp)
GAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCG32070.11266143044535518No Hit
CCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACAT30400.10679474541748667Illumina RNA RT Primer (95% over 22bp)
ACTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTAT29940.10517877229603784Illumina RNA RT Primer (95% over 22bp)
GCACCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA29550.10380870812785298Illumina RNA RT Primer (95% over 22bp)
ACACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGAC28480.10004981412796117Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)