FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846582
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG2053717.214652520110083RNA PCR Primer, Index 41 (96% over 29bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG531501.8671515522827025RNA PCR Primer, Index 41 (96% over 29bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA92250.3240728705514192RNA PCR Primer, Index 41 (96% over 29bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCT80610.283181724608671RNA PCR Primer, Index 41 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG74730.26252537253449926RNA PCR Primer, Index 41 (96% over 29bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG50760.1783191209668297RNA PCR Primer, Index 41 (96% over 29bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAA43450.1526392002759801RNA PCR Primer, Index 41 (96% over 29bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTT38560.13546070339796992RNA PCR Primer, Index 41 (96% over 28bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAA37730.1325449258092688RNA PCR Primer, Index 41 (96% over 29bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAA36280.12745109749165842RNA PCR Primer, Index 41 (96% over 29bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGT35030.12305986618337361RNA PCR Primer, Index 41 (96% over 29bp)
CCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA32220.11318837820234935RNA PCR Primer, Index 41 (96% over 29bp)
CTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA32210.11315324835188306RNA PCR Primer, Index 41 (96% over 29bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAA30480.10707578422121689RNA PCR Primer, Index 41 (96% over 29bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAA30460.10700552452028433RNA PCR Primer, Index 41 (96% over 29bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG29800.10468695438950995RNA PCR Primer, Index 41 (96% over 29bp)
GAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG29430.10338714992225764RNA PCR Primer, Index 41 (96% over 29bp)
ACTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA28720.10089293053915187RNA PCR Primer, Index 41 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)