FastQCFastQC Report
Wed 8 May 2024
EGAF00001682383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2929778
Total Bases219.7 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCC2278737.777824804473239Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGC605302.0660268457200512Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCG99030.338011958585258Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAG89750.3063372037062194Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTG83570.28524345530616996RNA PCR Primer, Index 41 (96% over 25bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATG58840.20083432942700777Illumina RNA RT Primer (95% over 22bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTC46230.1577935256527969Illumina RNA RT Primer (95% over 22bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGG43040.14690532866312736Illumina RNA RT Primer (95% over 22bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA42740.14588136029419294Illumina RNA RT Primer (95% over 22bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATC41460.14151242858673935Illumina RNA RT Primer (95% over 22bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATG35770.1220911618559495Illumina RNA RT Primer (95% over 22bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA35050.11963363777050685Illumina RNA RT Primer (95% over 22bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA34110.11642520354784561Illumina RNA RT Primer (95% over 22bp)
CCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTAT33490.11430900225204776Illumina RNA RT Primer (95% over 22bp)
GAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCG32990.1126023883038237No Hit
CTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTAT32820.11202213956142754Illumina RNA RT Primer (95% over 22bp)
ACTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTAT31090.10611725530057226Illumina RNA RT Primer (95% over 22bp)
CCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACAT31080.10608312302160779Illumina RNA RT Primer (95% over 22bp)
GCACCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA31010.10584419706885641Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)