FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2929778
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG2078757.095247489741544RNA PCR Primer, Index 41 (96% over 29bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG541801.849286874295595RNA PCR Primer, Index 41 (96% over 29bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA95870.32722615843248193RNA PCR Primer, Index 41 (96% over 29bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCT78380.26752880252360417RNA PCR Primer, Index 41 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG77090.26312573853718607RNA PCR Primer, Index 41 (96% over 29bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG50470.17226561193373696RNA PCR Primer, Index 41 (96% over 29bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAA44550.15205930278676405RNA PCR Primer, Index 41 (96% over 29bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGT42130.14379929127735958RNA PCR Primer, Index 41 (96% over 29bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAA39620.13523208925727478RNA PCR Primer, Index 41 (96% over 29bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTT39290.1341057240514469RNA PCR Primer, Index 41 (96% over 28bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAA38560.1316140676870398RNA PCR Primer, Index 41 (96% over 29bp)
CCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA32310.11028139333423898RNA PCR Primer, Index 41 (96% over 29bp)
CTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA31770.108438250270157RNA PCR Primer, Index 41 (96% over 29bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAA31520.10758494329604497RNA PCR Primer, Index 41 (96% over 29bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAA31450.10734601734329358RNA PCR Primer, Index 41 (96% over 29bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG30910.1055028742792116RNA PCR Primer, Index 41 (96% over 29bp)
ACTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA30040.10253336600930174RNA PCR Primer, Index 41 (96% over 29bp)
GAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG29690.10133873624554489RNA PCR Primer, Index 41 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)