FastQCFastQC Report
Wed 8 May 2024
EGAF00001682384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2876338
Total Bases215.7 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCC2260117.857595317379252Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGC599102.082856743539876Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCG96170.33434874482762456Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAG89630.3116115004564832Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTG79130.27510675031932963RNA PCR Primer, Index 41 (96% over 25bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATG57990.20161052004319382Illumina RNA RT Primer (95% over 22bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTC45880.15950837488501005Illumina RNA RT Primer (95% over 22bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGG42070.1462623655495286Illumina RNA RT Primer (95% over 22bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA42050.1461928326921245Illumina RNA RT Primer (95% over 22bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATC40920.14226422624879276Illumina RNA RT Primer (95% over 22bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATG35550.12359465403579135Illumina RNA RT Primer (95% over 22bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA33720.117232397583316Illumina RNA RT Primer (95% over 22bp)
CCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTAT33470.11636323686576473Illumina RNA RT Primer (95% over 22bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA33220.11549407614821347Illumina RNA RT Primer (95% over 22bp)
CTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTAT33140.11521594471859706Illumina RNA RT Primer (95% over 22bp)
GAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCG32010.11128733827526528No Hit
CCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACAT31120.10819312612078275Illumina RNA RT Primer (95% over 22bp)
GCACCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACA30140.10478601610798176Illumina RNA RT Primer (95% over 22bp)
ACTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTAT29170.10141367252388278Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)