FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2876338
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG2054317.142102214691041RNA PCR Primer, Index 41 (96% over 29bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG536371.86476693629191RNA PCR Primer, Index 41 (96% over 29bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA92830.3227367576411395RNA PCR Primer, Index 41 (96% over 29bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCT78000.27117814387599787RNA PCR Primer, Index 41 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG73160.25435119238420517RNA PCR Primer, Index 41 (96% over 29bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG49910.1735192456519366RNA PCR Primer, Index 41 (96% over 29bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAA43800.15227695771498342RNA PCR Primer, Index 41 (96% over 29bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAA38890.13520664122227638RNA PCR Primer, Index 41 (96% over 29bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTT38300.13315542192885538RNA PCR Primer, Index 41 (96% over 28bp)
GCCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAA37520.13044364049009538RNA PCR Primer, Index 41 (96% over 29bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGT37380.12995691048826669RNA PCR Primer, Index 41 (96% over 29bp)
CTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA32160.11180883470579604RNA PCR Primer, Index 41 (96% over 29bp)
CCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA32110.11163500256228578RNA PCR Primer, Index 41 (96% over 29bp)
ATGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG30680.10666340325789249RNA PCR Primer, Index 41 (96% over 29bp)
GACCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAA30590.10635050539957404RNA PCR Primer, Index 41 (96% over 29bp)
ACCCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAA30040.10443835182096124RNA PCR Primer, Index 41 (96% over 29bp)
GAATTCTCGGGTGCCAAGTCGACGTACGATCAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG28940.10061404466373562RNA PCR Primer, Index 41 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)