FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3671366
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAATAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG678161.8471598854486313TruSeq Adapter, Index 1 (96% over 27bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAATAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG185460.5051525780867394RNA PCR Primer, Index 40 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAATAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA44920.12235227977815341RNA PCR Primer, Index 40 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)