FastQCFastQC Report
Wed 8 May 2024
EGAF00001682389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089538
Total Bases306.7 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCC2115265.172369103796077Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGC544221.330761567688086Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTG65130.15926004355504217Illumina PCR Primer Index 6 (95% over 23bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATG53570.13099279185081544Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCG52610.12864533842209072Illumina RNA RT Primer (95% over 22bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGA45370.11094162714712517Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)