FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001682389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089538
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1800034.401548536778482Illumina PCR Primer Index 6 (96% over 27bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG438951.073348627644492Illumina PCR Primer Index 6 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG58610.14331692235162016Illumina PCR Primer Index 6 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGG49900.12201867301391991Illumina PCR Primer Index 6 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA48550.11871756662977578Illumina PCR Primer Index 6 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)