FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001682390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3994084
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1793844.49124254772809Illumina PCR Primer Index 6 (96% over 27bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG441131.1044584941127928Illumina PCR Primer Index 6 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG55120.13800410807584415Illumina PCR Primer Index 6 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA47100.11792441020268979Illumina PCR Primer Index 6 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGG45030.11274174504091553Illumina PCR Primer Index 6 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)