FastQCFastQC Report
Wed 8 May 2024
EGAF00001682391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4109989
Total Bases308.2 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCC2138965.204296167216019Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGC559341.3609282165962002Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTG65510.15939215409092336Illumina PCR Primer Index 6 (95% over 23bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCG54030.13146020585456555Illumina RNA RT Primer (95% over 22bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATG52960.12885679256075866Illumina RNA RT Primer (95% over 22bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGA46460.113041665075016Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)