FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001682391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4109989
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1822344.433929141902813Illumina PCR Primer Index 6 (96% over 27bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG456391.110440928187399Illumina PCR Primer Index 6 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG59310.14430695556606113Illumina PCR Primer Index 6 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA50010.12167915777876778Illumina PCR Primer Index 6 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGG46290.11262803866385043Illumina PCR Primer Index 6 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)