FastQCFastQC Report
Wed 8 May 2024
EGAF00001682392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4034658
Total Bases302.5 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCC2112175.235065772613193Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGC547621.3572897628497882Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTG61120.15148743710123636Illumina PCR Primer Index 6 (95% over 23bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATG52200.12937899569182817Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCG51530.12771838406130084Illumina RNA RT Primer (95% over 22bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGA43500.10781582974319012Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)