FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001682392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4034658
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1776474.403024989974367Illumina PCR Primer Index 6 (96% over 27bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG438811.0876014770025118Illumina PCR Primer Index 6 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG54760.13572401923533545Illumina PCR Primer Index 6 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGG51010.12642955115402596Illumina PCR Primer Index 6 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA47260.11713508307271644Illumina PCR Primer Index 6 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)