FastQCFastQC Report
Wed 8 May 2024
EGAF00001682393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216078
Total Bases241.2 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCC1968246.120000820875613Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGC520841.6194880845551631Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCG171930.5345952430258222Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAG80060.24893674842463398Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTG74480.23158642296610965RNA PCR Primer, Index 46 (100% over 23bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTC70490.21918000745006805Illumina RNA RT Primer (95% over 22bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATG50010.15549995988903254Illumina RNA RT Primer (95% over 22bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGC47910.14897026751216855Illumina RNA RT Primer (95% over 22bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATC35470.11028961362255518Illumina RNA RT Primer (95% over 22bp)
TACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAG34410.10699367366090003Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)