FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216078
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1719125.345392742340204Illumina PCR Primer Index 4 (96% over 27bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG432921.346111630377124Illumina PCR Primer Index 4 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA161390.5018224060486095Illumina PCR Primer Index 4 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG68060.21162422055683974Illumina PCR Primer Index 4 (96% over 27bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCT67190.20891906228642465Illumina PCR Primer Index 4 (96% over 25bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA65120.20248265122923015Illumina PCR Primer Index 4 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGG40110.12471712439810229Illumina PCR Primer Index 4 (96% over 27bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTT40010.12440618666587067Illumina PCR Primer Index 4 (96% over 26bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG38730.12042618369330595Illumina PCR Primer Index 4 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)