FastQCFastQC Report
Wed 8 May 2024
EGAF00001682394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140021
Total Bases235.5 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCC1936426.166901431550936Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGC511751.6297661703536377Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCG165890.5283085686369614Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAG78100.2487244512058996Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTG72820.23190927703986694RNA PCR Primer, Index 46 (100% over 23bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTC66540.21190941079693415Illumina RNA RT Primer (95% over 22bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATG52180.16617723257264838Illumina RNA RT Primer (95% over 22bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGC46530.1481837223381627Illumina RNA RT Primer (95% over 22bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATC33260.10592285847769807Illumina RNA RT Primer (95% over 22bp)
TACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAG32800.10445790012232402Illumina RNA RT Primer (95% over 22bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATATCGTATGCC31980.10184645261926593Illumina RNA RT Primer (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)