FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140021
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1695205.398690008761088Illumina PCR Primer Index 4 (96% over 27bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG428841.3657233502578485Illumina PCR Primer Index 4 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA155480.4951559241164311Illumina PCR Primer Index 4 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG65970.2100941363131011Illumina PCR Primer Index 4 (96% over 27bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCT65460.20846994335388205Illumina PCR Primer Index 4 (96% over 25bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA61580.19611333809550954Illumina PCR Primer Index 4 (96% over 27bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG39860.1269418261852389Illumina PCR Primer Index 4 (96% over 27bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTT38900.12388452179141478Illumina PCR Primer Index 4 (96% over 26bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGG35490.11302472180918537Illumina PCR Primer Index 4 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)