FastQCFastQC Report
Wed 8 May 2024
EGAF00001682395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3231737
Total Bases242.3 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCC1978496.122063769421831Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGC528941.6367049670192841Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCG174170.538936181997483Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAG79350.2455335938537078Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTG77700.24042798037092744RNA PCR Primer, Index 46 (100% over 23bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTC69470.2149617991810596Illumina RNA RT Primer (95% over 22bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATG53050.16415320924939128Illumina RNA RT Primer (95% over 22bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGC47940.14834127900878072Illumina RNA RT Primer (95% over 22bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATC34770.10758920048258877Illumina RNA RT Primer (95% over 22bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATATCGTATGCC34640.10718694002636972Illumina RNA RT Primer (95% over 21bp)
TACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAG33610.10399979948863412Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)