FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3231737
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1724645.336572870874083Illumina PCR Primer Index 4 (96% over 27bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG442741.3699753414340339Illumina PCR Primer Index 4 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA163720.5066006299398744Illumina PCR Primer Index 4 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG70890.21935572108745235Illumina PCR Primer Index 4 (96% over 27bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCT66380.20540037756785284Illumina PCR Primer Index 4 (96% over 25bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA64390.1992426982764996Illumina PCR Primer Index 4 (96% over 27bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG40730.1260312952446316Illumina PCR Primer Index 4 (96% over 27bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTT39610.12256566669874437Illumina PCR Primer Index 4 (96% over 26bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGG37380.11566535271898672Illumina PCR Primer Index 4 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGT32690.10115303318308388Illumina PCR Primer Index 4 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)