FastQCFastQC Report
Wed 8 May 2024
EGAF00001682396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176616
Total Bases238.2 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCC1956136.157905141823878Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGC523131.6468153531934613Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCG168960.5318867625170937Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAG79510.2502978011821385Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTG70990.22347680676543846RNA PCR Primer, Index 46 (100% over 23bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTC68460.21551235654545592Illumina RNA RT Primer (95% over 22bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATG51110.16089448645980503Illumina RNA RT Primer (95% over 22bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGC47240.14871171082686735Illumina RNA RT Primer (95% over 22bp)
CCCCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATC34610.1089524198077451Illumina RNA RT Primer (95% over 22bp)
TACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAG33210.10454521415241881Illumina RNA RT Primer (95% over 22bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATATCGTATGCC32580.10256197160752197Illumina RNA RT Primer (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)