FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176616
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1682785.297398237621419Illumina PCR Primer Index 4 (96% over 27bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG434131.3666429936762896Illumina PCR Primer Index 4 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA156730.49338667311377893Illumina PCR Primer Index 4 (96% over 27bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCT66040.20789418676982044Illumina PCR Primer Index 4 (96% over 25bp)
CGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG64010.20150373856959733Illumina PCR Primer Index 4 (96% over 27bp)
CCGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA62450.19659285226794804Illumina PCR Primer Index 4 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGG40900.12875336521631825Illumina PCR Primer Index 4 (96% over 27bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTT38760.12201663657174805Illumina PCR Primer Index 4 (96% over 26bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG38510.12122963556186835Illumina PCR Primer Index 4 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)