FastQCFastQC Report
Wed 8 May 2024
EGAF00001682401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3838997
Total Bases287.9 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCC2538296.611857211662317Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGC646221.683304258898874Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTG68530.17851016815069143TruSeq Adapter, Index 5 (95% over 23bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATG64300.16749166514066044Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCG63850.16631948396938054Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAC47730.12432934956708745Illumina RNA RT Primer (95% over 22bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGAGCTCGTATGCC46320.12065651523041043Illumina RNA RT Primer (95% over 21bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACT41970.1093254305747048Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)