FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3838997
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG2365936.162885774591645TruSeq Adapter, Index 5 (96% over 27bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG595121.5501965747824236RNA PCR Primer, Index 21 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG64520.16806473149106393TruSeq Adapter, Index 5 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA62630.16314157057168838RNA PCR Primer, Index 21 (96% over 27bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG57800.1505601593332842RNA PCR Primer, Index 21 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGT40420.10528791765140738TruSeq Adapter, Index 5 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG39640.10325613695452224Illumina Single End Adapter 2 (96% over 26bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCT39550.10302170072026627RNA PCR Primer, Index 21 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)