FastQCFastQC Report
Wed 8 May 2024
EGAF00001682402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736673
Total Bases280.2 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCC2493746.673690740399281Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGC642241.7187482019432796Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTG65800.17609247584682952TruSeq Adapter, Index 5 (95% over 23bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCG63560.17009783837119277Illumina RNA RT Primer (95% over 22bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATG62240.16656528414447827Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAC46840.12535215149947562Illumina RNA RT Primer (95% over 22bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGAGCTCGTATGCC40720.10897394553925377Illumina RNA RT Primer (95% over 21bp)
ACGCTATTGGAGCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACT40530.10846547182480244Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)