FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736673
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG2321116.211702228158578TruSeq Adapter, Index 5 (96% over 27bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG589921.5787305980480497RNA PCR Primer, Index 21 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA62360.16688642543781593RNA PCR Primer, Index 21 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG62090.16616385752780616TruSeq Adapter, Index 5 (96% over 27bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG56200.15040117237981487RNA PCR Primer, Index 21 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGT40810.10921480150925703TruSeq Adapter, Index 5 (96% over 27bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCT39850.10664567116255558RNA PCR Primer, Index 21 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)