FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3855969
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG2350546.095847762261574TruSeq Adapter, Index 5 (96% over 27bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG598801.552917048866316RNA PCR Primer, Index 21 (96% over 27bp)
CGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG64470.16719532755579725TruSeq Adapter, Index 5 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA61560.15964858638645693RNA PCR Primer, Index 21 (96% over 27bp)
CTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG57130.14815990481251276RNA PCR Primer, Index 21 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGT47100.12214828490581746TruSeq Adapter, Index 5 (96% over 27bp)
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG44140.11447187464422043Illumina Single End Adapter 2 (96% over 26bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAACTTGATCTCGTATGCCGTCTTCTGCT40900.10606931746598586RNA PCR Primer, Index 21 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)