FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001682408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4363980
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAATGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG348190.7978725842006609TruSeq Adapter, Index 2 (96% over 28bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAATGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG84400.19340143630355777Illumina PCR Primer Index 11 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)