FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001682415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5382858
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG472930.8785853165734634Illumina PCR Primer Index 5 (96% over 32bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG122520.2276114287242948Illumina PCR Primer Index 5 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)