FastQCFastQC Report
Wed 8 May 2024
EGAF00001682421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5071064
Total Bases380.3 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCCGATGTATCTCGTATGCC708711.3975568046469145Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCCGATGTATCTCGTATGC172930.3410132469241169Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)