FastQCFastQC Report
Wed 8 May 2024
EGAF00001682425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3955070
Total Bases296.6 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCTTAGGCATCTCGTATGCC954902.4143694043341837Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCTTAGGCATCTCGTATGC250230.6326815960273775Illumina RNA RT Primer (95% over 22bp)
AGACACTCCCACATGGTCTAGCGGTTAGGATTCCTGGAATTCTCGGGTGC47110.11911293605422912No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)