FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3865442
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG843802.1829327668090737Illumina PCR Primer Index 3 (96% over 32bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG219880.5688353362953059Illumina PCR Primer Index 3 (96% over 32bp)
AGACACTCCCACATGGTCTAGCGGTTAGGATTCCTGGAATTCTCGGGTGCCAAGTCGACGTACGATCTTAGGCAT42540.11005209753502962Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)