FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001682432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4599187
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG443650.9646270090779088Illumina PCR Primer Index 4 (96% over 32bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG115810.2518053734279559Illumina PCR Primer Index 4 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)