FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001682453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4687324
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG236590.5047442847987466Illumina PCR Primer Index 7 (96% over 32bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG55090.1175297461835367Illumina PCR Primer Index 7 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)