FastQCFastQC Report
Wed 8 May 2024
EGAF00001682461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3642603
Total Bases273.1 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCGATCAGATCTCGTATGCC688201.889308277624545Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCGATCAGATCTCGTATGC178740.490693056586183Illumina RNA RT Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)