FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001682462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3590051
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG618031.7215075774689552Illumina PCR Primer Index 9 (96% over 32bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG157090.43757038549034544Illumina PCR Primer Index 9 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)