FastQCFastQC Report
Wed 8 May 2024
EGAF00001682481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4001003
Total Bases300 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCC1141052.8519098835966883Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGC325830.8143707965227719Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAA87370.2183702436614019Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCG83620.2089975938533413Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCCGTCTTCTG56050.14008987246447954TruSeq Adapter, Index 4 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)