FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001682481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4001003
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1053672.633514646202465TruSeq Adapter, Index 4 (96% over 28bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG296590.7412891217527205Illumina PCR Primer Index 12 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA81510.20372391622800584Illumina PCR Primer Index 12 (96% over 27bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCCGTCTTCTGCT78060.19510107840459004Illumina PCR Primer Index 12 (96% over 25bp)
CGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG52880.1321668591600656TruSeq Adapter, Index 4 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)