FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001682482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3943188
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1050062.6629721940724105TruSeq Adapter, Index 4 (96% over 28bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG293910.7453613675026399Illumina PCR Primer Index 12 (96% over 27bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA78000.19780948816034133Illumina PCR Primer Index 12 (96% over 27bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCCGTCTTCTGCT77920.19760660663402305Illumina PCR Primer Index 12 (96% over 25bp)
CGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG50220.12735887814631208TruSeq Adapter, Index 4 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)