FastQCFastQC Report
Wed 8 May 2024
EGAF00001682483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4018904
Total Bases301.4 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCC1156572.8778244018767305Illumina RNA RT Primer (95% over 21bp)
TGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGC330600.8226123341090009Illumina RNA RT Primer (95% over 22bp)
TTCCCTACACGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAA89210.22197594169952803Illumina RNA RT Primer (95% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCG83030.20659861494576628Illumina RNA RT Primer (95% over 22bp)
CGGGTGCCAAGTCGACGTACGATCAAAGCAATCTCGTATGCCGTCTTCTG57010.14185459518316437TruSeq Adapter, Index 4 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)