FastQCFastQC Report
Thu 9 May 2024
EGAF00001682505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37758003
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGATCTCGTA25432746.735721695874647Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGAGCTCGTA710340.18812965293741835Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGAACTCGTA596910.1580883395766455Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGACCTCGTA502620.13311615023707687Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG392120.10385083130588235Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)