FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001682505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001682505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37758003
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA24515096.492687126488125Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA566450.150021175643214Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA472990.12526880725127332Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA391760.10375548728040516Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)