FastQCFastQC Report
Thu 23 Feb 2023
EGAF00001685231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001685231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103331477
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC4019620.3890024721121522TruSeq Adapter, Index 5 (100% over 50bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1364090.13201108119261665No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT1187990.11496883955312087No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1084710.10497382128777662No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC1076600.10418896847859825No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)