FastQCFastQC Report
Wed 15 Feb 2023
EGAF00001685259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001685259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111662616
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCA7668410.686748195116618No Hit
GGGTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAG4299570.38505008695121384No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT3786130.33906871750165696No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA3605960.3229335053371846No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA3321120.2974245203067784No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT3238210.2899994748466219No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG3188690.2855646871106799No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA3177480.2845607700969499No Hit
GTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGAC2870660.2570833554535387No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2767520.24784660248332352No Hit
CGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCA2691840.24106904319705352No Hit
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGA2598420.2327027695643455No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC2587730.23174542140406237No Hit
CTCCGTTCATGGTGATGCTGTCCACGGTGATCTGCCAGTAACCCTCGACG2483810.22243881515367686No Hit
CTCGACGGTAACAGGCACCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGT2169940.19433003432411078No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT2111800.18912327828679923No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2069310.1853180656272642No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA2020210.1809208911960293No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2012270.18020982062609028No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1984800.1777497313872711No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1944820.1741693030011047No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1909990.17105008537503724No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA1899830.170140201623075No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1870570.16751980806181366No Hit
GTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCA1857960.16639051336572663No Hit
GTGGGCACAGGACAGAGGCCAGATCTTCCTGGGAGGTGGCTGAAGAGACT1826000.16352831998849104No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1816430.16267127397409353No Hit
CCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGTCAATGCCACCAAAGATC1793330.16060254221520298No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC1784250.15978937838963042No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT1768320.15836275947538253No Hit
GTTGGCAATGGGGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGGTGTCAA1739490.15578087477370223No Hit
GCCGATAGTGCCGAAGTACTCCATATCCAGGTAGTTCTCCAGGGGCTGTT1698640.15212253311349969No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC1626290.14564319360026456No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG1565110.14016418888126353No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGAT1556700.13941102723224755No Hit
GTCCAGAGTCCAGAAAAAGAAGGCTGAATCCAGAGTGGAAAGACAGATAC1550110.13882085657029564No Hit
GTCAGAGATGCCTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCA1528110.13685063584754276No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC1507430.13499862836815502No Hit
CTCGCTGGCTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGC1467110.13138775111627332No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT1391550.1246209384884911No Hit
GGAGAATTAGATACATCTAAAGTGGGCACAGGACAGAGGCCAGATCTTCC1390980.1245698918606743No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC1380040.12359015482854173No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT1346860.12061870375668075No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1326020.11875236739930936No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC1304140.1167928933350442No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG1302680.11666214232344332No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA1270370.1137686045256185No Hit
GGCAGGAGTTCCGATGCCGATAGTGCCGAAGTACTCCATATCCAGGTAGT1235380.1106350580215674No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA1222220.10945650780741158No Hit
GTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCAC1216990.10898813260832077No Hit
CCAGGATCCAAAGCTCTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAG1209210.10829139091636542No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1205050.10791884008879032No Hit
CCTGGTTCCAGATGTTGTCAAAGACGGGTGTGGCCCCGGAGGAGGAAATG1202940.10772987801038084No Hit
CTGGTTGTTTGCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACAT1178720.10556084410560469No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT1164520.1042891561845551No Hit
CAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGAACAGTCAGGA1155690.10349838123083199No Hit
GCCAGGTTCCGTCTCGCTCAGGCCGAAGATCTGATTGGTGTCAGAGATGC1152770.10323687920763024No Hit
CTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCATGCTGCCGGTG1133990.10155502715429846No Hit
CAGGAGAATTAGATACATCTAAAGTGGGCACAGGACAGAGGCCAGATCTT1129100.1011171008209229No Hit
CATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCAGG1127100.10093798984612719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)