FastQCFastQC Report
Thu 9 Feb 2023
EGAF00001685313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001685313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122977801
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT2786050.22654901757431814TruSeq Adapter, Index 19 (97% over 40bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2424220.19712663426141439No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1734250.14102138645331608No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1277740.10390005266072369No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)